Maked liquid medium.
Yeast extract 0.5 g
Peptone 1 g
NaCl 1 g
Amp 100 µl
According to protocol.
Did not sterilize before autoclave, just in the bottle.
Add Amp and use for pre-culture.
Maked agarose gel.
Agarose 1.2 g
TAE Buffer 100 ml
1. Melted agarose and TAE Buffer in conical flask by microwave oven.
2. Poured into a mould pierced a comb.
3. After set kept in TAE Buffer.
Pre cultured colony of GFP + OriTr.
1. Maked liquid medium.
2. Checked transformation.
-> J23100 and K317037 were grown.
3. Plasmid extraction
-> K283030 extracted. Keep the extract in the coldroom in Cell-biochemistry lab.
4. Pre culture
Each of J23100 and K317037 cultured three.
They have been incubating at 37 deg C.