Team:Tokyo Metropolitan/Notebook/S05
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- | '''-Molecular Genetics Microbiology-''' | + | ='''-Molecular Genetics Microbiology-'''= |
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. |
Revision as of 04:53, 5 October 2011
-Molecular Genetics Microbiology-
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI