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Team:Osaka/week7
From 2011.igem.org
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[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
| - | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10 | + | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]] |
Revision as of 12:52, 2 October 2011
Contents |
September 11(Sun)
- Gel extraction of digests
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
- Transformation of ligation products
September 12(Mon)
- To liquid culture:103,104,105,106
September 13(Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 107(A):12(upstream)+103(downstream)+1-1G(vector)
- 108(A):12(upstream)+104(downstream)+1-1G(vector)
- Transformation of ligation products
September 14(Wed)
- To liquid culture:107,108
September 15(Thu)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:108
September 16(Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
September 17(Sat)
- Ligation
- 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
- 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
- 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
- 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
- Transformation of ligation products
