Team:Panama/7 June 2011

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(Created page with "we prepared:  500ml LB medium  Autoclaving LB medium  Glycerol 30% for preservation  Check the medium with plasmids, colonies grows well; negative control doesn’t ...")
 
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we prepared:
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We prepared:
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 500ml LB medium
 500ml LB medium
 +
 Autoclaving LB medium
 Autoclaving LB medium
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 Glycerol 30% for preservation  
+
 
 +
 Glycerol 30% for preservation
 +
 Check the medium with plasmids, colonies grows well; negative control doesn’t grow after 22h on incubation.
 Check the medium with plasmids, colonies grows well; negative control doesn’t grow after 22h on incubation.
 +
 We inoculate bacterias with plasmids P+R, R+T and with Rh in liquid LB + Amp. Incubate at 37oC in shaker.
 We inoculate bacterias with plasmids P+R, R+T and with Rh in liquid LB + Amp. Incubate at 37oC in shaker.

Latest revision as of 04:24, 29 September 2011

We prepared:

 500ml LB medium

 Autoclaving LB medium

 Glycerol 30% for preservation

 Check the medium with plasmids, colonies grows well; negative control doesn’t grow after 22h on incubation.

 We inoculate bacterias with plasmids P+R, R+T and with Rh in liquid LB + Amp. Incubate at 37oC in shaker.

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