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User contributions
From 2011.igem.org
- 04:14, 29 September 2011 (diff | hist) N Team:Panama/19 September 2011 (Created page with "We did a PCR and an electrophoresis of the miniprep products (for probe if someone of these colonies had our insert). We did the mutagenesis with the Quick Change Lighting Multi-...") (top)
- 04:10, 29 September 2011 (diff | hist) N Team:Panama/18 September 2011 (Created page with "We had to much BACTERIAL COLONIES with our insert, and we did quick check too all the colonies we could!") (top)
- 04:07, 29 September 2011 (diff | hist) N Team:Panama/17 September 2011 (Created page with "We inoculate the rh1AB+pGEM-T's colonies on liquid LB-media.") (top)
- 04:05, 29 September 2011 (diff | hist) N Team:Panama/15 September 2011 (Created page with "We did an electrophoresis of the ligation (rh1AB+pGEM-T)") (top)
- 04:04, 29 September 2011 (diff | hist) N Team:Panama/14 September 2011 (Created page with "We did another cloning with rh1AB and pGEM-T (pGEM T-easy and pGEM-T Cloning Kit)") (top)
- 04:03, 29 September 2011 (diff | hist) N Team:Panama/13 September 2011 (Created page with "The cells with pGEM-T plasmids didn't grew, we'll do an electrophoresis of the ligation and a plasmidic extraction of the chloramphenicol's cultures (for probe if someone has chl...") (top)
- 03:59, 29 September 2011 (diff | hist) N Team:Panama/12 September 2011 (Created page with "Results: Which Cell is competent? A= E. coli k-12 and E. coli JM109 (MgCl2) Which strain has chloramphenicol's resistance? A= E. coli k-12 and E. coli JM109 have chloramphenicol'...") (top)
- 03:48, 29 September 2011 (diff | hist) Team:Panama/10 September 2011 (top)
- 03:44, 29 September 2011 (diff | hist) Team:Panama/9 September 2011 (top)
- 03:42, 29 September 2011 (diff | hist) N Team:Panama/10 September 2011 (Created page with "We prepared 5 mL of chloramphenicol and 5 mL of ampicilin, the chloramphenicol was stored on -20 centigrades and the ampicilin was stored on 4 centigrades. We prepared 14 petri d...")
- 03:26, 29 September 2011 (diff | hist) N Team:Panama/9 September 2011 (Created page with "We observed the transformations, our results are the following: Petri Dishes with growht: LB+chloramphenicol+inducible Promoter, LB+Ampicilin+Inducible Promoter, LB+Ampicilin+C+...")
- 03:19, 29 September 2011 (diff | hist) N Team:Panama/6 September 2011 (Created page with "We inoculate, bacterial colonies, on LB media: the sample 8 with an ampicilin's control")
- 02:57, 29 September 2011 (diff | hist) N Team:Panama/28 June 2011 (Created page with "We have problems because negative control have grows in Lb medium + Chloranfenicol Two of tree petri dish with bacterias with Rh1AB grows; in the third one grow less colonies tha...") (top)
- 02:56, 29 September 2011 (diff | hist) N Team:Panama/27 June 2011 (Created page with "Today, we transform the Rh1AB gene, do it an electrophoresis of (P+RBS) and (REP+TER) from overnight samples.") (top)
- 02:53, 29 September 2011 (diff | hist) N Team:Panama/24 June 2011 (Created page with "No one tubes show any sign of grow. We believe that the cells are not competent, therefore, we have to do the transformation again.") (top)
- 02:53, 29 September 2011 (diff | hist) N Team:Panama/20 June 2011 (Created page with "We spread the strain with our gene on liquid LB medium + Chloranfenicol then incubate for the next day.") (top)
- 02:53, 29 September 2011 (diff | hist) N Team:Panama/17 June 2011 (Created page with "Yami is going to do a homemade miniprep for Rh gene but it can be possible because nothings grow. Why nothing grows? Because we use the wrong buffer.") (top)
- 02:52, 29 September 2011 (diff | hist) N Team:Panama/15 June 2011 (Created page with "Orlando will go to prepare miniprep for strains with P + RBS and R + T. Homemate miniprep protocol.") (top)
- 02:52, 29 September 2011 (diff | hist) N Team:Panama/16 June 2011 (Created page with " We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination. Orlando prepare chloranfenicol (100µg/ml) We pass...")
- 02:51, 29 September 2011 (diff | hist) N Team:Panama/9 June 2011 (Created page with "Transform bacterias with P+R was inoculate in brand new petri dishes with LB medium+ Amp, also prepare Glycerol stock solution of P+R and R-T then save in freezer at -800C") (top)
- 02:49, 29 September 2011 (diff | hist) N Team:Panama/8 June 2011 (Created page with "We check tubes was incubate yesterday")
- 02:48, 29 September 2011 (diff | hist) N Team:Panama/7 June 2011 (Created page with "we prepared: 500ml LB medium Autoclaving LB medium Glycerol 30% for preservation Check the medium with plasmids, colonies grows well; negative control doesn’t ...")
- 02:47, 29 September 2011 (diff | hist) N Team:Panama/6 June 2011 (Created page with "Today, mainly do it competent cells transformation incorporated plasmids P+R (promoting+RBS) and T+R (reporter+terminator), we do it the same procedure of five days ago (1/6/11) ...") (top)
- 02:45, 29 September 2011 (diff | hist) Team:Panama/1 June 2011 (top)
- 02:44, 29 September 2011 (diff | hist) N Team:Panama/1 June 2011 (Created page with "We did the transformation of P+R and G+T with negative controls. Transformation protocol material: Ice PCR tubes EPP tubes Thermocycler SOC medium Micropipette peaks For the pr...")
- 05:56, 25 September 2011 (diff | hist) Team:Panama/TheTeam (→Students)
- 05:52, 25 September 2011 (diff | hist) N File:Sergio-mosquera.jpg (top)
