Team:Panama/Protocols/Wetlab
From 2011.igem.org
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7. [[Ampicillin stock]] | 7. [[Ampicillin stock]] | ||
- | 8.[[ | + | 8.[[Chloramphenicol stock]] |
9. [[Mutagenesis PCR]] | 9. [[Mutagenesis PCR]] |
Revision as of 20:36, 28 September 2011
Wetlab
1. Agarose gel electrophoresis3. Colony PCR
4. Pgem Cloning
5. Preparing chemically competent cells
10. Mutagenesis transformation
12. Miniprep "Dirty"
13. PCR purification
14. Glycerol Stock
15. PCR
16. Agar plates
17. Primer aliquots
18. Cell Culture