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Revision as of 22:41, 27 September 2011

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== Digest of ho1 and Cph8 == Project: Regulatory Date: Wed, 03 Aug 2011 16:44:51 GMT Author: Nathan Davis (davis950) Access: Public Revision History:

• Wed, 03 Aug 2011 16:47:49 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Double digest of ho1 and Cph8 set up and placed in 37 degree C incubator. Reaction mixture was as follows:

• 3 ul NEBuffer 3 (100% activity for both BglII and NotI)

• 1 ul BglII

• 1 ul NotI (acquired from Claudia's lab)

• 10 ul template DNA

• 16 ul ddH2O

Digestion should be run for 24 hours and quenched by heating at 65 degree C for 20 minutes.

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