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Team:Colombia/Notebook/Protocols
From 2011.igem.org
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| - | + | ===PCR CBP=== | |
{| border="1" align="left" style="text-align:center;" | {| border="1" align="left" style="text-align:center;" | ||
|Reactives | |Reactives | ||
|1X µl | |1X µl | ||
|3X µl | |3X µl | ||
| + | |- | ||
|H2O | |H2O | ||
|18.8 | |18.8 | ||
|56.4 | |56.4 | ||
| + | |- | ||
|Buffer PFX | |Buffer PFX | ||
|2.5 | |2.5 | ||
|7.5 | |7.5 | ||
| + | |- | ||
|MgSO4 | |MgSO4 | ||
|0.5 | |0.5 | ||
|1.5 | |1.5 | ||
| + | |- | ||
|dNTP´s | |dNTP´s | ||
|0.75 | |0.75 | ||
|2.25 | |2.25 | ||
| + | |- | ||
|FW (Pr3) | |FW (Pr3) | ||
|0.75 | |0.75 | ||
|2.25 | |2.25 | ||
| + | |- | ||
|RV (Pr4) | |RV (Pr4) | ||
|0.75 | |0.75 | ||
|2.25 | |2.25 | ||
| + | |- | ||
|PFX | |PFX | ||
|0.2 | |0.2 | ||
|0.6 | |0.6 | ||
| + | |- | ||
|ADN | |ADN | ||
|0.75 | |0.75 | ||
|2.25 | |2.25 | ||
| + | |- | ||
|Mix Volume | |Mix Volume | ||
|25 | |25 | ||
|75 | |75 | ||
|} | |} | ||
Revision as of 16:36, 27 September 2011
Template:Https://2011.igem.org/User:Tabima
Contents |
Protocols
PCR for 16s (10 µl)
| Reactives | µl | µl |
| Buffer | 2.5 | 1 |
| MgCl2 | 2 | 0.8 |
| DNTP´s | 0.5 | 0.2 |
| P1492R | 0.5 | 0.2 |
| P27F | 0.5 | 0.2 |
| Taq | 0.25 | 0.1 |
| DNA | 2 | 0.8 |
| H2O | 17.75 | 6.8 |
| Final Vol | 26 | 10 |
PCR Pfx
| Reactives | ul |
| H2O | 17.2 |
| Buffer PFX | 3 |
| MgSO4 | 0.6 |
| dNTP´s | 0.9 |
| FW | 0.9 |
| RV | 0.9 |
| Enhancer | 3 |
| Taq PFX | 0.5 |
| ADN | 3 |
| Total Mix | 30 |
PCR CBP
| Reactives | 1X µl | 3X µl |
| H2O | 18.8 | 56.4 |
| Buffer PFX | 2.5 | 7.5 |
| MgSO4 | 0.5 | 1.5 |
| dNTP´s | 0.75 | 2.25 |
| FW (Pr3) | 0.75 | 2.25 |
| RV (Pr4) | 0.75 | 2.25 |
| PFX | 0.2 | 0.6 |
| ADN | 0.75 | 2.25 |
| Mix Volume | 25 | 75 |
