Team:Colombia/Notebook/Protocols

From 2011.igem.org

(Difference between revisions)
(→PCR for 16s (10 µl))
(→PCR for 16s (10 µl))
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|10
|10
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|}
 +
 +
===PCR Pfx===
 +
|Reactives
 +
|ul
 +
|-
 +
|H2O
 +
|17.2
 +
|-
 +
|Buffer PFX
 +
|3
 +
|-
 +
|MgSO4
 +
|0.6
 +
|-
 +
|dNTP´s
 +
|0.9
 +
|-
 +
|FW
 +
|0.9
 +
|-
 +
|RV
 +
|0.9
 +
|-
 +
|Enhancer
 +
|3
 +
|-
 +
|Taq PFX
 +
|0.5
 +
|-
 +
|ADN
 +
|3
 +
|-
 +
|Total Mix
 +
|30

Revision as of 16:16, 27 September 2011

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10

PCR Pfx

|Reactives |ul |- |H2O |17.2 |- |Buffer PFX |3 |- |MgSO4 |0.6 |- |dNTP´s |0.9 |- |FW |0.9 |- |RV |0.9 |- |Enhancer |3 |- |Taq PFX |0.5 |- |ADN |3 |- |Total Mix |30

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