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Team:Colombia/Notebook/Protocols
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(Created page with "===PCR for 16s (10 µl)=== {| border="1" align="center" style="text-align:center;" |Reactives |µl |µl |Buffer |2.5 |1 |MgCl2 |2 |0.8 |DNTP´s |0.5 |0.2 |P1492R |0.5 |0.2 |P27F...")
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(Created page with "===PCR for 16s (10 µl)=== {| border="1" align="center" style="text-align:center;" |Reactives |µl |µl |Buffer |2.5 |1 |MgCl2 |2 |0.8 |DNTP´s |0.5 |0.2 |P1492R |0.5 |0.2 |P27F...")
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Revision as of 16:11, 27 September 2011
PCR for 16s (10 µl)
| Reactives | µl | µl | Buffer | 2.5 | 1 | MgCl2 | 2 | 0.8 | DNTP´s | 0.5 | 0.2 | P1492R | 0.5 | 0.2 | P27F | 0.5 | 0.2 | Taq | 0.25 | 0.1 | DNA | 2 | 0.8 | H2O | 17.75 | 6.8 | Total Mix | 26 | 10 |
