"
Team:Washington/Protocols
From 2011.igem.org
(Difference between revisions)
(→Break It: Gluten Destruction Protocols) |
|||
| Line 44: | Line 44: | ||
=Break It: Gluten Destruction Protocols= | =Break It: Gluten Destruction Protocols= | ||
| - | [https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Cell Lysate Assay] | + | [https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay] |
[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification] | [https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification] | ||
[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Purified Enzyme Assay] | [https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Purified Enzyme Assay] | ||
| - | |||
=Make It: iGem Toolkits= | =Make It: iGem Toolkits= | ||
Revision as of 01:05, 23 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGem Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)
