Team:ETH Zurich/Process/Microfluidics

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Revision as of 23:58, 21 September 2011

Can you feel the smoke tonight?

Microfluidics

First Channel Designs

Final Channel Design

We relatively early figured out that we need a some kind of channel to establish the acetaldehyde or xylene gradient needed for SmoColi (see Circuit Design). However, there were several different channel designs possible, and the final design evolved through an iterative series of design steps and design validations. The first designs were validated based on vast simulations, the final design furthermore by biological experiments in the lab (see Systems Validation).

First Channel Designs

Microfluidic channel with flow and recycling of the medium

- Variant 1: Plate with pixel filled with agarose and cell and a microfluidic channel above
- Variant 2: Microfluidic channel with cells sitting in pockets in the channel

Final Channel Design

Microfluidic channel without flow

Experimental setup for SmoColi.

The Modeling showed that diffusion and degradation of acetaldehyde/ xylene is enough to create a concentration gradient in the tube. Without a flow there is no need for a liquid so we decided to fill the whole channel with agarose and cells likewise we don´t need recycling because AHL can diffuse through the whole channel.

@@ Line 5: / Line 5: @@
 {|class="processTitle linkMap" align="right"
 |-
-|style="border-left: none;"|[[#Final Experimental Setup|Final Experimental Setup]]
+|style="border-left: none;"|[[#First Channel Designs|First Channel Designs]]
-|[[#B|B]]
+|[[#Final Channel Design|Final Channel Design]]
 |}
 |-
 |colspan="2"|
-'''Insert text here'''.
+'''We relatively early figured out that we need a some kind of channel to establish the acetaldehyde or xylene gradient needed for SmoColi (see [[Team:ETH_Zurich/Overview/CircuitDesign|Circuit Design]]). However, there were several different channel designs possible, and the final design evolved through an iterative series of design steps and design validations. The first designs were validated based on vast simulations, the final design furthermore by biological experiments in the lab (see [[Process/Validation|Systems Validation]]).'''
 |}
 {|class="roundContainer"
 |
-== '''Evolution of channel design''' ==
+== '''First Channel Designs''' ==
-We relatively early figured out that we need a some kind of channel to establish the acetaldehyde or xylene gradient needed for SmoColi (see [[Team:ETH_Zurich/Overview/CircuitDesign|Circuit Design]]). However, there were several different channel designs possible, and the final design evolved through an iterative series of design steps and design validations. The first designs were validated based on vast simulations, the final design furthermore by biological experiments in the lab (see [[Validation|Systems Validation]]).
+==='''Microfluidic channel with flow and recycling of the medium'''===
-#'''Microfluidic channel with flow and recycling of the medium'''
 #* Variant 1: Plate with pixel filled with agarose and cell and a microfluidic channel above
 #* Variant 2: Microfluidic channel with cells sitting in pockets in the channel
-#'''Microfluidic channel without flow'''
+|}
+{|class="roundContainer"
+|
+=='''Final Channel Design'''==
+==='''Microfluidic channel without flow'''===
 [[File:ETH_final_setup_sensor.png|400px|right|thumb|'''Experimental setup for SmoColi.''' ]]
 :: The Modeling showed that diffusion and degradation of acetaldehyde/ xylene is enough to create a concentration gradient in the tube. Without a flow there is no need for a liquid so we decided to fill the whole channel with agarose and cells likewise we don´t need recycling because AHL can diffuse through the whole channel.
 |}