Team:ETH Zurich/Process/Microfluidics

From 2011.igem.org

(Difference between revisions)

Revision as of 20:55, 21 September 2011

Can you feel the smoke tonight?

Microfluidics

Final Experimental Setup

B

Insert text here.

Evolution of channel design

Microfluidic channel with flow and recycling of the medium
- Variant 1: Plate with pixel filled with agarose and cell and a microfluidic channel above
- Variant 2: Microfluidic channel with cells sitting in pockets in the channel
Microfluidic channel without flow

Experimental setup for SmoColi.

The Modeling showed that diffusion and degradation of acetaldehyde/ xylene is enough to create a concentration gradient in the tube. Without a flow there is no need for a liquid so we decided to fill the whole channel with agarose and cells likewise we don´t need recycling because AHL can diffuse through the whole channel.

@@ Line 14: / Line 14: @@
 {|class="roundContainer"
 |
-== '''Final experimental setup''' ==
+== '''Evolution of channel design''' ==
-'''Evolution of channel design'''
-# Microfluidic channel with flow and recycling of the medium
-## Plate with pixel filled with agarose and cell and a microfluidic channel above
+#'''Microfluidic channel with flow and recycling of the medium'''
-## Microfluidic channel with cells sitting in pockets in the channel
+#* Variant 1: Plate with pixel filled with agarose and cell and a microfluidic channel above
-# Microfluidic channel without flow
+#* Variant 2: Microfluidic channel with cells sitting in pockets in the channel
-[[File:ETH_final_setup_sensor.png|600px|center|thumb|'''Experimental setup for SmoColi.''' ]]
+#'''Microfluidic channel without flow'''
+[[File:ETH_final_setup_sensor.png|400px|right|thumb|'''Experimental setup for SmoColi.''' ]]
+:: The Modeling showed that diffusion and degradation of acetaldehyde/ xylene is enough to create a concentration gradient in the tube. Without a flow there is no need for a liquid so we decided to fill the whole channel with agarose and cells likewise we don´t need recycling because AHL can diffuse through the whole channel.
 |}