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Team:DTU-Denmark/Project
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[[File:DTU2011_modeling_fig.png|200px|frameless|right|'''Kinetic models''' of the system are the basis for modeling. Blue is target mRNA, red is small RNA and green is trap-RNA]] | [[File:DTU2011_modeling_fig.png|200px|frameless|right|'''Kinetic models''' of the system are the basis for modeling. Blue is target mRNA, red is small RNA and green is trap-RNA]] | ||
| - | A '''framework''' for characterization was developed to guide rational design and test hypotheses. '''Steady state analysis''' revealed that each trap-RNA system has a characteristic fold repression | + | A '''framework''' for characterization was developed to guide rational design and test hypotheses. '''Steady state analysis''' revealed that each trap-RNA system has a characteristic fold repression. |
[[Team:DTU-Denmark/Modeling|Read more...]] | [[Team:DTU-Denmark/Modeling|Read more...]] | ||
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== Data == | == Data == | ||
Revision as of 16:04, 21 September 2011
Project: Overview
Contents |
Abstract
Small regulatory RNA is an active area of research with untapped possibilities for application in biotechnology. Such applications include convenient gene silencing and fine-tuning of gene expression, which are currently cumbersome processes restricted to well studied bacteria. We have investigated a novel type of RNA regulation, where the inhibition caused by a small regulatory RNA is relieved by another RNA called trap-RNA. We explore the possibility of using the system to uniquely target and repress any gene of interest providing unprecedented specificity and control of gene silencing.
Bioinformatic
A bioinformatic study was performed to investigate the possibilities of engineering the trap-RNA system to target any gene. The study elucidates interesting features of sequence and secondary structure conservation guiding future application.
Experiments 1
Verifying that the envisioned small RNA based gene silencing is possible. Plasmids containing and strains deleted for the components were constructed providing a biological model.
Experiments 2
The dynamic range of the araBAD promoter was expanded.
Modeling
A framework for characterization was developed to guide rational design and test hypotheses. Steady state analysis revealed that each trap-RNA system has a characteristic fold repression.
Data
The data page provides a description of the constructed BioBricks and how they work.
Sponsors
Thanks to:
