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Team:DTU-Denmark/Project
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| + | Small regulatory RNA is an active area of research with untapped possibilities for application in biotechnology. Such applications include convenient gene silencing and fine-tuning of gene expression, which are currently cumbersome processes restricted to well studied bacteria. We have investigated a novel type of RNA regulation, where the inhibition caused by a small regulatory RNA is relieved by another RNA called trap-RNA. We explore the possibility of using the system to uniquely target and repress any gene of interest providing unprecedented specificity and control of gene silencing. | ||
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<iframe style="float: right; margin: 5px 0 20px 10px; border: 4px solid white; outline: 1px solid #CCCCCC;" width="450" height="300" src="http://www.youtube.com/embed/dtleYPzOg-Y" frameborder="0" allowfullscreen></iframe> | <iframe style="float: right; margin: 5px 0 20px 10px; border: 4px solid white; outline: 1px solid #CCCCCC;" width="450" height="300" src="http://www.youtube.com/embed/dtleYPzOg-Y" frameborder="0" allowfullscreen></iframe> | ||
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[[File:DTU2011_project_fig1.png|100px|frameless|right|Two-level sRNA regulation. Blue is any target mRNA, red is sRNA and green is trap-RNA.]] | [[File:DTU2011_project_fig1.png|100px|frameless|right|Two-level sRNA regulation. Blue is any target mRNA, red is sRNA and green is trap-RNA.]] | ||
Revision as of 16:01, 21 September 2011
Project: Overview
Contents |
Abstract
Small regulatory RNA is an active area of research with untapped possibilities for application in biotechnology. Such applications include convenient gene silencing and fine-tuning of gene expression, which are currently cumbersome processes restricted to well studied bacteria. We have investigated a novel type of RNA regulation, where the inhibition caused by a small regulatory RNA is relieved by another RNA called trap-RNA. We explore the possibility of using the system to uniquely target and repress any gene of interest providing unprecedented specificity and control of gene silencing.
Bioinformatic
A bioinformatic study was performed to investigate the possibilities of engineering the trap-RNA system to target any gene. The study elucidates interesting features of sequence and secondary structure conservation guiding future application.
Experiments 1
Verifying that the envisioned small RNA based gene silencing is possible. Plasmids containing and strains deleted for the components were constructed providing a biological model.
Experiments 2
The dynamic range of the araBAD promoter was expanded.
Modeling
A framework for characterization was developed to guide rational design and test hypotheses. Steady state analysis revealed that each trap-RNA system has a characteristic fold repression. For more information got to modeling.
Data
The data page provides a description of the constructed BioBricks and how they work.
Sponsors
Thanks to:
