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Team:ETH Zurich/Process/Validation
From 2011.igem.org
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To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution. | To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution. | ||
| - | [[File:Setup_test.png| | + | [[File:Setup_test.png|300px|left|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]] |
[[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in tube''' E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.]] | [[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in tube''' E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.]] | ||
Revision as of 13:42, 21 September 2011
| Systems Validation |
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System validation for diffusionTo test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.   |
