Team:ETH Zurich/Process/Validation

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[[File:Setup_test.png|600px|center|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]]
[[File:Setup_test.png|600px|center|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]]
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[[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in a 2mm tube''' E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.]]
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[[File:ETHZ Gradient.png|800px|center|thumb|'''GFP gradient in tube''' E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.]]

Revision as of 13:40, 21 September 2011

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Systems Validation
Diffusion-only System A
Text goes here

System validation for diffusion

To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.

Experimental setup for the diffusion test in agarose filled tubes.
GFP gradient in tube E.coli with IPTG ineducable GFP were incubated in a tube (2 mm diameter, 7 cm long). GFP expression was assessed under the fluorescent microscope after over night with a excitation wavelength of 480 nm and a emission wavelength of 510 nm.
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