"
Team:ETH Zurich/Process/Validation
From 2011.igem.org
(Difference between revisions)
(→Test diffusion with IPTG induced GFP) |
(→System validation for diffusion) |
||
| Line 17: | Line 17: | ||
To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution. | To test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution. | ||
[[File:Setup_test.png|600px|center|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]] | [[File:Setup_test.png|600px|center|thumb|'''Experimental setup for the diffusion test in agarose filled tubes.''' ]] | ||
| + | |||
| + | [[File:ETHZ Gradient.png|300px|center|thumb|]] | ||
Results | Results | ||
Revision as of 13:31, 21 September 2011
| Systems Validation |
| ||
| Text goes here | |||
System validation for diffusionTo test if we can create a gradient of a small molecule along our agarose filled tube. We filled a tube with agarose and E. coli with IPTG inducible GFP was incubated at 37 °C overnight on end of the tube sticking in 1 ml of 10 mM IPTG solution.   Results
|
