Team:UNIPV-Pavia

From 2011.igem.org

(Difference between revisions)
Line 39: Line 39:
-
<div style="width:700px;margin:10px auto;">
+
<div style="width:500px;margin:10px auto;">
<div class="slider-wrapper theme-default">
<div class="slider-wrapper theme-default">
<div class="ribbon"></div>
<div class="ribbon"></div>
-
<div id="nivoslider-125" class="nivoSlider" style="width:700px;height:470px;">
+
<div id="nivoslider-125" class="nivoSlider" style="width:500px;height:470px;">
<img src="https://static.igem.org/mediawiki/2011/8/81/UNIPVteam1.jpg" title="UNIPV iGEM team" alt="" width="500"/>
<img src="https://static.igem.org/mediawiki/2011/8/81/UNIPVteam1.jpg" title="UNIPV iGEM team" alt="" width="500"/>

Revision as of 07:41, 18 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


You can add captions too…



Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia"