Team:Washington/Protocols

From 2011.igem.org

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[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]

Revision as of 22:36, 15 September 2011


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagensis

[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Cell Lysate Assay by Decarbonylase Redesign Team


Break It: Gluten Destruction Protocols

Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification


Make It: iGem Toolkits

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Induction Studies of Proteins Fusions (sfGFP)

PBS Stock Protocol

Preparation of Overnight Cultures

Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)

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