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SEPTEMBER: WEEK 3
September, 12th
E31-1, E41N-1, E42-1, E43-3 and ENTERO4C5 were diluted 1:100 in 13 falcon tubes (4ml M9 + Cm12.5 pH = 6.0). After two hours they were induced with aTc (6 ng/ml, 8 ng/ml and 100 ng/ml). Supernatants were taken at the induction time, after 1 hour, 2 hours and 4 hours.
September, 13thSupernatants collected on September, 12th were tested, measuring 3OC6-HSL concentration with T9002-ENTERO in two differents tests.1:100 dilution of E37-2, E38-1, E39-1, E40-2 and ENTERO4C5 in 4ml M9 + Cm12.5 and two hours growth, after which cultures were induced with aTc; addition of 3OC6-HSL 100 nM after one hour and collection of supernatants at t = 0h, t = 1 h, t = 2 h and t = 4 h (time refers to 3OC6-HSL addition). Inoculum of E44 for plasmid purification in 5 ml LB + Cm34; inoculum of T9002-ENTERO and ENTERO-RBS for 3OC6-HSL concentration measurement; ENTERO4C5 was inoculated in M9 + Cm12.5 at pH = 6.0 and pH = 7.0 in order to detect 3OC6-HSL degradation in culutres not expressing AiiA. September, 14thSeptember, 15thSeptember, 16thSeptember, 17thSeptember, 18th
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Team:UNIPV-Pavia/Calendar/September/week3
From 2011.igem.org
(Difference between revisions)
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<a name="September.2C_13th"></a><h2> <span class="mw-headline">September, 13th</span></h2> | <a name="September.2C_13th"></a><h2> <span class="mw-headline">September, 13th</span></h2> | ||
| - | + | Supernatants collected on <a href="#September.2C_12th">September, 12th</a> were tested, measuring 3OC<sub><small>6</small></sub>-HSL concentration with T9002-ENTERO in two differents tests. | |
| + | <br> | ||
| + | 1:100 dilution of E37-2, E38-1, E39-1, E40-2 and ENTERO4C5 in 4ml M9 + Cm12.5 and two hours growth, after which cultures were induced with aTc; addition of 3OC<sub><small>6</small></sub>-HSL 100 nM after one hour and collection of supernatants at t = 0h, t = 1 h, t = 2 h and t = 4 h (time refers to 3OC<sub><small>6</small></sub>-HSL addition). | ||
| + | <br> | ||
| + | Inoculum of E44 for plasmid purification in 5 ml LB + Cm34; inoculum of T9002-ENTERO and ENTERO-RBS for 3OC<sub><small>6</small></sub>-HSL concentration measurement; ENTERO4C5 was inoculated in M9 + Cm12.5 at pH = 6.0 and pH = 7.0 in order to detect 3OC<sub><small>6</small></sub>-HSL degradation in culutres not expressing AiiA. | ||
| + | </p> | ||
<div align="right"><small><a href="#indice" title="">^top</a></small></div> | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
Revision as of 14:12, 15 September 2011
