"
Team:Washington/Protocols
From 2011.igem.org
(Difference between revisions)
(→Make It: Magnetosome Protocols) |
m |
||
| Line 38: | Line 38: | ||
[https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team] | [https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team] | ||
| + | |||
=Break It: Gluten Destruction Protocols= | =Break It: Gluten Destruction Protocols= | ||
| Line 65: | Line 66: | ||
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | [https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | ||
| + | |||
=Wiki Design= | =Wiki Design= | ||
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | [https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | ||
Revision as of 17:15, 14 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: Magnetosome Protocols
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)
