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Team:Washington/Protocols
From 2011.igem.org
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=Break It: Gluten Destruction Protocols= | =Break It: Gluten Destruction Protocols= | ||
Revision as of 04:22, 14 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: Magnetosome Protocols
Electroporation (Transformation)
Isolation of Plasmid DNA (miniprep)
Preparation of Overnight Cultures
