Team:UNIPV-Pavia/Tecan Measurements

From 2011.igem.org

(Difference between revisions)
Line 9: Line 9:
<p style="text-align:left;">
<p style="text-align:left;">
-
 
+
<p>
-
 
+
<ul type="circle">
-
 
+
<li><b>04-08-2011</b> preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
-
 
+
<li><b>05-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
-
 
+
<li><b>17-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
-
 
+
<li><b>18-08-2011</b> PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
 +
<li><b>20-08-2011</b> PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
 +
<li><b>23-08-2011</b> the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
 +
<li><b>24-08-2011</b> Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
 +
<li><b>25-08-2011</b> Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
 +
<li><b>26-08-2011</b> preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
 +
<li><b>29-08-2011</b> preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
 +
<li><b>03-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
 +
<li><b>05-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
 +
<li><b>05-09-2011</b> test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd
 +
<li><b>06-09-2011</b> test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th
 +
<li><b>07-09-2011</b> test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th)
 +
</ul>
 +
</p>
</html>
</html>
{{endcalendar}}
{{endcalendar}}

Revision as of 21:41, 7 September 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

TECAN MEASUREMENTS

  • 04-08-2011 preliminary test on PtetR (E32, E33, E34, E35 induced with 0, 1, 10, 50, 100 ng/ml atc)
  • 05-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 3rd
  • 17-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc, diluted to 1:200 and 1:500 in microplate) SN collected on August, 3rd
  • 18-08-2011 PtetR with RBS30 and RBS31 (E32, E33 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 20-08-2011 PtetR with RBS32 and RBS34 (E34, E35 induced with 0, 1, 2, 3, 4, 5, 8, 10, 50, 100 ng/ml atc) x 2
  • 23-08-2011 the day TECAN broke down! (We should have performed a test on SN of AiiA LC E37, E38, E39, E40 induced with 75 ng/ml atc)
  • 24-08-2011 Plux with RBS30 and RBS31 (E17, E18 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 25-08-2011 Plux with RBS32 and RBS34 (E19, E20 induced with 0, 0.1, 0.5, 1, 2, 5, 10, 100 nM HSL) x 3
  • 26-08-2011 preliminary test on AiiA LC (E37, E38, E39, E40 induced with 75 ng/ml atc) SN collected on August, 19th
  • 29-08-2011 preliminary test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on August, 27th
  • 03-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h
  • 05-09-2011 test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
  • 05-09-2011 test on AiiA HC in TOP10 (E24, E25, E26, E27, not induced) SN collected on Sptember, 3rd
  • 06-09-2011 test on luxI producing HSL (E31, E41, E42, E43 induced with 100 ng/ml atc) SN collected on September, 5th
  • 07-09-2011 test on AiiA LC (E37, E38, E39, E40 induced with 100 ng/ml atc) SN collected on September, 6th)

University of Pavia | Faculty of Engineering | Biomedical Informatics

Copyright © 2011. All Rights Reserved.

Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia/Tecan_Measurements"