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Team:ETH Zurich/Modeling/SingleCell
From 2011.igem.org
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The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed). | The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed). | ||
| - | Acetaldehyde from the smoke binds to AlcR and the formed complex then inhibits TetR. TetR inhibits LacI and CI. CI also inhibits LacI which inhibits GFP. This part of the circuit makes up the band detector. The Acetaldehyde-AlcR complex is considered as constant input to our system. By varying | + | ==Brief overview of the model== |
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| + | Acetaldehyde from the smoke binds to AlcR and the formed complex then inhibits TetR. TetR inhibits LacI and CI. CI also inhibits LacI which inhibits GFP. This part of the circuit makes up the band detector. The Acetaldehyde-AlcR complex is considered as constant input to our system. By simulating with varying acetaldehyde concentrations we can see at which concentration is the band , i.e. at which concentrations GFP is produced. The connection to the switch is done by the | ||
=Band Detector= | =Band Detector= | ||
Revision as of 15:26, 6 September 2011
The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed).
Brief overview of the model
Acetaldehyde from the smoke binds to AlcR and the formed complex then inhibits TetR. TetR inhibits LacI and CI. CI also inhibits LacI which inhibits GFP. This part of the circuit makes up the band detector. The Acetaldehyde-AlcR complex is considered as constant input to our system. By simulating with varying acetaldehyde concentrations we can see at which concentration is the band , i.e. at which concentrations GFP is produced. The connection to the switch is done by the
Band Detector
Toggle Switch
