"
Team:Freiburg/Notebook/5 September
From 2011.igem.org
(Difference between revisions)
(→Miniprep) |
(→Miniprep) |
||
| Line 43: | Line 43: | ||
continue from experiment: Transformation; 03.09.11 <br/> | continue from experiment: Transformation; 03.09.11 <br/> | ||
testdigest showed no inserts | testdigest showed no inserts | ||
| - | |||
| + | ===Trafo=== | ||
| + | |||
| + | '''Investigators. Sandra''' | ||
| + | |||
| + | Trafo of: | ||
| + | #pGEX vector (Amp. resistance) | ||
| + | #pGEX vector (Amp. resistance) | ||
| + | #pGEX vector (Amp. resistance) | ||
| + | #pSB1C3 | ||
| + | #pET Duet vector (Amp. resistance) | ||
| + | #pET Duet vector (Amp. resistance) | ||
| + | #pET Duet vector (Amp. resistance) | ||
| + | #pSB1C3 | ||
| + | #pSB1C3 | ||
| + | #pSB1C3 | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
===Trafo=== | ===Trafo=== | ||
Revision as of 10:49, 6 September 2011
Contact 
This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
transformation with reporter parts
Investigators:Julia
transformation with iGEM parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15016 BBa_I15016] which is B0032.ECFP
and
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I15017 BBa_I15017 ]which is B0032.EYFP.
Tomorrow the green light promotor from PCR of 29_August should be inserted infront of the reporter proteins CFP and YFP.
blue light receptor
Miniprep
Investigators:Sophie
continue from: picking clones; 04.09.11
Testdigest showed no inserts
Ligation
Investigators: Sophie
again ligation as last time (02.08.11)
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Miniprep
Investigators: Sophie
continue from experiment: Transformation; 03.09.11
testdigest showed no inserts
Trafo
Investigators. Sandra
Trafo of:
- pGEX vector (Amp. resistance)
- pGEX vector (Amp. resistance)
- pGEX vector (Amp. resistance)
- pSB1C3
- pET Duet vector (Amp. resistance)
- pET Duet vector (Amp. resistance)
- pET Duet vector (Amp. resistance)
- pSB1C3
- pSB1C3
- pSB1C3
Trafo
Investigators. Sandra
Repeat of the trafo of:
