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Team:ETH Zurich/Modeling/SingleCell
From 2011.igem.org
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The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed). | The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed). | ||
| - | + | Acetaldehyde from the smoke binds to AlcR and the formed complex then inhibits TetR. TetR inhibits LacI and CI. CI also inhibits LacI which inhibits GFP. This part of the circuit makes up the band detector. The Acetaldehyde-AlcR complex is considered as constant input to our system. By varying its concentration we can see | |
=Band Detector= | =Band Detector= | ||
Revision as of 10:00, 6 September 2011
The model of our system consists of two connected parts: the band detector and the toggle switch. The band detector produces green fluorescent protein upon a detection of a certain range (band) of acetaldehyde concentration. After acetaldehyde concentration passes a certain threshold the toggle switch turns the system red (red fluorescent protein is expressed).
Acetaldehyde from the smoke binds to AlcR and the formed complex then inhibits TetR. TetR inhibits LacI and CI. CI also inhibits LacI which inhibits GFP. This part of the circuit makes up the band detector. The Acetaldehyde-AlcR complex is considered as constant input to our system. By varying its concentration we can see
Band Detector
Toggle Switch
