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SEPTEMBER: WEEK 2
September, 5th
T9002-ENTERO and ENTERO-4C5 were diluted 1:200 and 1:800 to perform two different tests on supernatants (collected on August, 31st, from cultures and media at different pHs, and on September, 3rd, from cultures expressing AiiA enzyme in high copy number plasmids).
September, 6thSeptember, 7thSeptember, 8thSeptember, 9thSeptember, 10thSeptember, 11th
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Team:UNIPV-Pavia/Calendar/September/week2
From 2011.igem.org
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<a name="September.2C_5th"></a><h2> <span class="mw-headline">September, 5th</span></h2> | <a name="September.2C_5th"></a><h2> <span class="mw-headline">September, 5th</span></h2> | ||
<p> | <p> | ||
| - | T9002-ENTERO and ENTERO-4C5 were diluted 1:200 and 1:800 to perform two different tests on supernatants (collected | + | T9002-ENTERO and ENTERO-4C5 were diluted 1:200 and 1:800 to perform two different tests on supernatants (collected on <a href="https://2011.igem.org/Team:UNIPV-Pavia/Calendar/August/week5#August.2C_31st">August, 31st</a>, from cultures and media at different pHs, and on <a href="https://2011.igem.org/Team:UNIPV-Pavia/Calendar/September/week1#September.2C_3rd">September, 3rd</a>, from cultures expressing AiiA enzyme in high copy number plasmids). |
<br> | <br> | ||
Samples of dry DNA were prepared for R0040 in J61002-1C3-1 and J101-31-1C3-1 and sent to BMR genomics for sequencing. | Samples of dry DNA were prepared for R0040 in J61002-1C3-1 and J101-31-1C3-1 and sent to BMR genomics for sequencing. | ||
<br> | <br> | ||
E31-1, E41N-1, E42-1, E43-2, E43-3 were washed in 1 ml of M9 + Cm12.5 in order to remove 3OC<small><sub>6</sub></small>-HSL due to PtetR leakage; then they were diluted 1:100 in 4 ml volume (falcon tube). After two hours they were induced with a final concentration of 100 ng/ml atc. Supernatants were collected at the moment of induction, named t = 0 h and then at t = 2 h, t = 4 h and t = 6 h. | E31-1, E41N-1, E42-1, E43-2, E43-3 were washed in 1 ml of M9 + Cm12.5 in order to remove 3OC<small><sub>6</sub></small>-HSL due to PtetR leakage; then they were diluted 1:100 in 4 ml volume (falcon tube). After two hours they were induced with a final concentration of 100 ng/ml atc. Supernatants were collected at the moment of induction, named t = 0 h and then at t = 2 h, t = 4 h and t = 6 h. | ||
| + | <br> | ||
| + | E3N-1C3 was again transformed in 200 μl of TOP10 competent cells. | ||
</p> | </p> | ||
Revision as of 14:10, 5 September 2011
