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Team:Osaka/week3
From 2011.igem.org
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# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
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[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
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| + | week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|'''3'''|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]|[[Team:Osaka/week14|14]] | ||
Revision as of 14:47, 21 September 2011
Contents |
August 16(Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18(Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-6I | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
August 19(Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction of 1-1G,1-3A
- Transfer to LB culture medium:1-6I,07
August 20(Sat)
- Gel electrophoresis of 1-5A
- Gel extraction of 1-5A
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
week 1|2|3|4|5|6|7|8|9|10|11|12|13|14
