Team:UNIPV-Pavia/Calendar/August/week5

From 2011.igem.org

(Difference between revisions)
Line 21: Line 21:
-
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width: 550px;"><a href="/File:UNIPV_29_08_2011_E43-3%28EP%29.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/1/10/UNIPV_29_08_2011_E43-3%28EP%29.png" class="thumbimage" height="80%" width="80%"></a>  <div class="thumbcaption">Small size gel</div></div></div></div>
+
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width: 550px;"><a href="/File:UNIPV_29_08_2011_E43-3%28EP%29.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/1/10/UNIPV_29_08_2011_E43-3%28EP%29.png" class="thumbimage" height="50%" width="50%"></a>  <div class="thumbcaption">Small size gel</div></div></div></div>
<p>
<p>
Plates incubated on saturday were all grown, except for J101-31-1C3; for each of them a colony was picked, while for J101-E7 two colonies were inoculated in LB + Cm34 5 ml.
Plates incubated on saturday were all grown, except for J101-31-1C3; for each of them a colony was picked, while for J101-E7 two colonies were inoculated in LB + Cm34 5 ml.
 +
<br>
 +
E43-2 was inoculated in order to screen again the insert length with gel electrophoresis.
 +
</p>
<div align="right"><small><a href="#indice" title="">^top</a></small></div>
<div align="right"><small><a href="#indice" title="">^top</a></small></div>
 +
<a name="August.2C_30th"></a><h2> <span class="mw-headline">August, 30th</span></h2>
 +
 +
<p>
 +
 +
 +
 +
 +
<div align="right"><small><a href="#indice" title="">^top</a></small></div>
 +
<div>
<div>

Revision as of 21:18, 1 September 2011

UNIPV TEAM 2011

March
M T W T F S S
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31    

April
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30

May
M T W T F S S
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31

June
M T W T F S S
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30

July
M T W T F S S
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31

August
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31

September
M T W T F S S
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30

October
M T W T F S S
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

AUGUST: WEEK 5

August, 29th

T9002-ENTERo and ENTERO-RBS were diluted 1:500 in M9 + Amp + Cm12.5 13 ml and 1 ml volume respectively. After six hour growth they were aliquoted in 96-well microplate (190 μl cultures) with 10 μl of supernatants collected on August, 27th and 10 μl of 3OC6-HSL.
5 flasks were prepared with 29.6 ml H2O, 200 μl glycerol 80 %, 8 ml M9 salts 5 x and their pH was adjusted to 5.9, 6.3, 6.7, 6.9 and 7.2; they were autoclaved in order to prepare M9 glycerol supplemented minimal medium.
E43-3 DNA was digested with Ecori and PstI restriction nucleases in order to screen the part length by gel-electrophoresis; it showed the correct band.

Small size gel

Plates incubated on saturday were all grown, except for J101-31-1C3; for each of them a colony was picked, while for J101-E7 two colonies were inoculated in LB + Cm34 5 ml.
E43-2 was inoculated in order to screen again the insert length with gel electrophoresis.

August, 30th