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AUGUST: WEEK 5
August, 29th
T9002-ENTERo and ENTERO-RBS were diluted 1:500 in M9 + Amp + Cm12.5 13 ml and 1 ml volume respectively. After six hour growth they were aliquoted in 96-well microplate (190 μl cultures) with 10 μl of supernatants collected on August, 27th and 10 μl of 3OC6-HSL.
 Small size gel Plates incubated on saturday were all grown, except for J101-31-1C3; for each of them a colony was picked, while for J101-E7 two colonies were inoculated in LB + Cm34 5 ml. 
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Team:UNIPV-Pavia/Calendar/August/week5
From 2011.igem.org
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E43-3 DNA was digested with Ecori and PstI restriction nucleases in order to screen the part length by gel-electrophoresis; it showed the correct band. | E43-3 DNA was digested with Ecori and PstI restriction nucleases in order to screen the part length by gel-electrophoresis; it showed the correct band. | ||
</p> | </p> | ||
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| + | <div class="center"><div class="thumb tnone"><div class="thumbinner" style="width: 550px;"><a href="/File:UNIPV_29_08_2011_E43-3%28EP%29.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/1/10/UNIPV_29_08_2011_E43-3%28EP%29.png" class="thumbimage" height="80%" width="80%"></a> <div class="thumbcaption">Small size gel</div></div></div></div> | ||
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| + | <p> | ||
| + | Plates incubated on saturday were all grown, except for J101-31-1C3; for each of them a colony was picked, while for J101-E7 two colonies were inoculated in LB + Cm34 5 ml. | ||
<div align="right"><small><a href="#indice" title="">^top</a></small></div> | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
Revision as of 21:15, 1 September 2011
