"
Team:Edinburgh/Experiments
From 2011.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
==4 August: ''malS'' starch degradation assay== | ==4 August: ''malS'' starch degradation assay== | ||
| - | + | Starch turns black in the presence of iodine, so we can do a qualitative test for starch degradation by flooding a starch agar plate with iodine. If starch degradation is occurring, we would expect to see a zone of clearing around the bacterial colonies. | |
| - | This is sort of expected. The ''malS'' amylase is periplasmic and so is not expected to actually degrade starch until we get it fused to INP so that it is present on the outside of the cell. | + | Colonies with the ''malS'' gene under the control of the lac promoter (we hope; awaiting sequencing) did not show starch degradation. This is sort of expected. The ''malS'' amylase is periplasmic and so is not expected to actually degrade starch until we get it fused to INP so that it is present on the outside of the cell. |
'''Fixme:''' this should be redone with proper controls. | '''Fixme:''' this should be redone with proper controls. | ||
Revision as of 09:52, 4 August 2011
4 August: malS starch degradation assay
Starch turns black in the presence of iodine, so we can do a qualitative test for starch degradation by flooding a starch agar plate with iodine. If starch degradation is occurring, we would expect to see a zone of clearing around the bacterial colonies.
Colonies with the malS gene under the control of the lac promoter (we hope; awaiting sequencing) did not show starch degradation. This is sort of expected. The malS amylase is periplasmic and so is not expected to actually degrade starch until we get it fused to INP so that it is present on the outside of the cell.
Fixme: this should be redone with proper controls.
