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AUGUST: WEEK 1
August, 1stFor each plate from the previous day, two colonies were picked. Next, MGZ1 competent cells were prepared according to protocol. They were put in 22 100 μl test-tubes.
Screening (E-S) bande 1-2-3. August, 2nd
MODIFICARE: una piccata per ciascuno degli J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate) Plasmids containing E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E28AGAIN-2, E37-2, E38-1. E39-1, E40-2 and E42-1 were extracted with MiniPrep kit from ON saturated cultures; purified DNA was quantified with NanoDrop Spectrophotometer:
MODIFICARE?: E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E42AGAIN-2, J101-31, J101-E5, J101-E7 were transformed in MGZ1 competent cells. Digestions of previously purified plasmids were performed for ligations. For each part (E28AGAIN-1, E28AGAIN-2, E41AGAIN-1 and E42AGAIN-2), the following aliquots for the various components were used:
MODIFICARE?: TOP10 competent cells, carrying T9002 construct, were transformed with ENTERO4C5.
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Team:UNIPV-Pavia/Calendar/August/week1
From 2011.igem.org
(Difference between revisions)
| Line 32: | Line 32: | ||
<p> | <p> | ||
| - | MODIFICARE: | + | MODIFICARE: una piccata per ciascuno degli J101-31, J101-E5, J101-E7 (DOMENICA 31, mentre SABATO 30 trasformate) |
</p> | </p> | ||
| Line 94: | Line 94: | ||
</table> | </table> | ||
</center> | </center> | ||
| + | |||
| + | <p> | ||
| + | MODIFICARE?: E28AGAIN-1, E28AGAIN-2, E41AGAIN-1, E42AGAIN-2, J101-31, J101-E5, J101-E7 were transformed in MGZ1 competent cells. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Digestions of previously purified plasmids were performed for ligations. For each part (E28AGAIN-1, E28AGAIN-2, E41AGAIN-1 and E42AGAIN-2), the following aliquots for the various components were used: | ||
| + | </p> | ||
| + | |||
| + | <center> | ||
| + | <table border="1"> | ||
| + | <tr> | ||
| + | <td><b>DNA (μl)</b></td> | ||
| + | <td><b>H<small><sub>2</sub></small>O (μl)</b></td> | ||
| + | <td><b>Enzyme 1 (μl)</b></td> | ||
| + | <td><b>Enzyme 2 (μl)</b></td> | ||
| + | <td><b>Buffer H (μl)</b></td> | ||
| + | <td><b>Final Volume (μl)</b></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td>16.5</td> | ||
| + | <td>0.5 EcoRI</td> | ||
| + | <td>0.5 Pstl</td> | ||
| + | <td>2.5</td> | ||
| + | <td>25</td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | </center> | ||
| + | |||
| + | |||
| + | <p> | ||
| + | MODIFICARE?: TOP10 competent cells, carrying T9002 construct, were transformed with ENTERO4C5. | ||
| + | </p> | ||
<table border="0" width="100%" class="menu"> | <table border="0" width="100%" class="menu"> | ||
Revision as of 09:59, 3 August 2011
