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Thursday, July 21
From 2011.igem.org
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| + | ==Tube Prep== | ||
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity. | Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity. | ||
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*R3 | *R3 | ||
*R4 | *R4 | ||
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| + | ===Incubation=== | ||
| + | The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday. | ||
{{LabReportBmore}} | {{LabReportBmore}} | ||
Revision as of 00:08, 22 July 2011
Tube Prep
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.
These tubes were from the Promoter Plate
- P1
- P2
- P3
- P4
These tubes were from the Terminator Plate
- T1
- T2
- T3
- T4
These tubes were from the Ribosome Binding Site Plate
- R1
- R2
- R3
- R4
Incubation
The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.
Hours
