"
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JULY: WEEK 2
July, 4th
Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols. In the afternoon gel electrophoresis was performed.
As shown, in figure all clones were positive (apart from <partinfo>BBa_I13501</partinfo> run where we inexplicably didn't see any band), so we cut and purified the bands of interest. Cells harbouring <partinfo>BBa_I13501</partinfo> were again inoculated in 5 ml LB + Amp. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Reactions were incubated ON at 16°C.
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Team:UNIPV-Pavia/Calendar/July/settimana2
From 2011.igem.org
(Difference between revisions)
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_C0060 | + | <td></html><partinfo>BBa_C0060</partinfo><html> (E-S)</td> |
<td>3.9</td> | <td>3.9</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_C0061 | + | <td></html><partinfo>BBa_C0061</partinfo><html> (X-P)</td> |
<td>4.1</td> | <td>4.1</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_K081022 | + | <td></html><partinfo>BBa_K081022)</partinfo><html> (E-P</td> |
<td>4.4</td> | <td>4.4</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_B0030 | + | <td></html><partinfo>BBa_B0030</partinfo><html> (S-P)</td> |
<td>10.3</td> | <td>10.3</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_B0031 | + | <td></html><partinfo>BBa_B0031</partinfo><html> (S-P)</td> |
<td>11.8</td> | <td>11.8</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_B0032 | + | <td></html><partinfo>BBa_B0032</partinfo><html> (S-P)</td> |
<td>10.1</td> | <td>10.1</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>BBa_B0015 | + | <td></html><partinfo>BBa_B0015</partinfo><html> (E-X)</td> |
<td>12</td> | <td>12</td> | ||
</tr> | </tr> | ||
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<tr> | <tr> | ||
| - | <td></html><partinfo>pSB4C5 | + | <td></html><partinfo>pSB4C5</partinfo><html> (E-P)</td> |
<td>10.7</td> | <td>10.7</td> | ||
</tr> | </tr> | ||
Revision as of 16:41, 16 July 2011
