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JULY: WEEK 2
July, 4th
Digestions of previously purified plasmids were performed for ligations:
Reactions were incubated at 37°C for three hours while a small-size and a medium-size agarose gel were prepared according to protocols. In the afternoon gel electrophoresis was performed.
As shown, in figure all clones were positive (apart from <partinfo>BBa_I13501</partinfo> run where we inexplicably didn't see any band), so we cut and purified the bands of interest. Cells harbouring <partinfo>BBa_I13501</partinfo> were again inoculated in 5 ml LB + Amp. After gel extraction, cut DNA was quantified:
Then ligations were performed in a final volume of 10 μl:
Reactions were incubated ON at 16°C.
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Team:UNIPV-Pavia/Calendar/July/settimana2
From 2011.igem.org
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<td>6.5</td> | <td>6.5</td> | ||
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<td>6.5</td> | <td>6.5</td> | ||
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<td>6.5</td> | <td>6.5</td> | ||
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<td></html><partinfo>BBa_C0061</partinfo><html> (X-P)</td> | <td></html><partinfo>BBa_C0061</partinfo><html> (X-P)</td> | ||
<td>6.5</td> | <td>6.5</td> | ||
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<td></html><partinfo>BBa_K081022</partinfo><html> (E-P)</td> | <td></html><partinfo>BBa_K081022</partinfo><html> (E-P)</td> | ||
<td>6.5</td> | <td>6.5</td> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
</center> | </center> | ||
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| + | Reactions were incubated ON at 16°C. | ||
| + | </p> | ||
<div align="right"><small><a href="#indice" title="">^top</a></small></div> | <div align="right"><small><a href="#indice" title="">^top</a></small></div> | ||
Revision as of 16:36, 16 July 2011
