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Team:EPF-Lausanne/Protocols
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* [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture. | * [[Team:EPF-Lausanne/Protocols/Miniprep|Plasmid preparation - Miniprep]]: recover plasmids from a bacterial culture. | ||
* [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | * [[Team:EPF-Lausanne/Protocols/TetR|Linear template- TetR]]. | ||
| + | * [[Team:EPF-Lausanne/Protocols/TetR Mutation PCR|tetR mutation PCR]]: induce site-specific mutations on tetR. | ||
* [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | * [[Team:EPF-Lausanne/Protocols/|Agarose gel electrophoresis]]: DNA samples analysis (can be followed by band purification). | ||
* [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells|Competent Cells]]. | ||
* [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | * [[Team:EPF-Lausanne/Protocols/Competent cells. Protocol II|Competent Cells. Protocol II (Inoue method)]]. | ||
* [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | * [[Team:EPF-Lausanne/Protocols/Transformation|Transformation]]. | ||
| - | * [[Team:EPF-Lausanne/Protocols/ | + | * [[Team:EPF-Lausanne/Protocols/Primer preparation|Primers preparation]]. |
* [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | * [[Team:EPF-Lausanne/Protocols/Gibson assembly|Gibson assembly]]. | ||
* [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]. | * [[Team:EPF-Lausanne/Protocols/Gel purification|Gel purification]]. | ||
Revision as of 14:18, 13 July 2011
Protocols
Molecular Biology
- Plasmid preparation - Miniprep: recover plasmids from a bacterial culture.
- Linear template- TetR.
- tetR mutation PCR: induce site-specific mutations on tetR.
- Agarose gel electrophoresis: DNA samples analysis (can be followed by band purification).
- Competent Cells.
- Competent Cells. Protocol II (Inoue method).
- Transformation.
- Primers preparation.
- Gibson assembly.
- Gel purification.
Microfluidics
- PDMS two layer device fabrication
- MITOMI: Protein – DNA interactions
- Chemostat cell culture
- Klenow dsDNA synthesis
