SmoColi-Data page

SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works

Our Favorite New Parts

1. [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacI_M1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI_M1 binds to P_lac and inhibits transcription. (Characterization)
2. [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. (Characterization)
3. [http://partsregistry.org/Part:BBa_K625003 MainPage]-P_U promoter BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)

Characterized New Parts

1. [http://partsregistry.org/Part:BBa_K625001 MainPage]- P_Tet - LacI_M1 - Termination, BBa_K625001 (Characterization)
2. [http://partsregistry.org/Part:BBa_K625002 MainPage]-P_U promoter, BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)

Characterized Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I13453:Experience Experience]-P_BAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P_BAD in BW27783 (Characterization)