Team:Harvard/Judging

From 2011.igem.org

(Difference between revisions)
(→Foundational Advances)
Line 19: Line 19:
=Accomplishments=
=Accomplishments=
-
 
-
 
==Experimental Results:==
==Experimental Results:==
'''✓''' Researched zinc finger proteins and choose 6 novel, <html><x>clinically relevant target sequences </x></html><br>
'''✓''' Researched zinc finger proteins and choose 6 novel, <html><x>clinically relevant target sequences </x></html><br>
Line 41: Line 39:
'''✓''' Handed out <html><x>IP pamphlets and chassis data sheets</x></html> at our poster presentation <br>
'''✓''' Handed out <html><x>IP pamphlets and chassis data sheets</x></html> at our poster presentation <br>
'''✓''' <html><x>Educated</x></html> local high school students about synthetic biology to provide accurate information from sources other than the media.
'''✓''' <html><x>Educated</x></html> local high school students about synthetic biology to provide accurate information from sources other than the media.
 +
Our <html><x>foundational advance</x></html> is how we <html><x>brought together novel technologies</x></html> to create a pipeline that allows scientists <html><x>to engineer novel interactions with DNA</x></html>. This is a new way of making new parts: using selection on a computationally designed library. The <html><x>genome is the next frontier</x></html> in synthetic biology, and we want the iGEM and synthetic biology communities to use these techniques: we have provided detailed protocols on using lambda red and MAGE.
Our <html><x>foundational advance</x></html> is how we <html><x>brought together novel technologies</x></html> to create a pipeline that allows scientists <html><x>to engineer novel interactions with DNA</x></html>. This is a new way of making new parts: using selection on a computationally designed library. The <html><x>genome is the next frontier</x></html> in synthetic biology, and we want the iGEM and synthetic biology communities to use these techniques: we have provided detailed protocols on using lambda red and MAGE.

Revision as of 22:10, 28 October 2011

bar

Wiki Highlights

HARVshield logo.png
  • Read our project description and summary on our Project page: more details on how team members completed work for the three sections of our project are on Design, Synthesize, and Test pages, respectively.
  • Please be sure to stop by our poster during the poster session with any questions.

Accomplishments

Experimental Results:

✓ Researched zinc finger proteins and choose 6 novel, clinically relevant target sequences
✓ Generated 55,000 zinc finger protein sequences using bioinformatics
✓ Used three recently developed technologies together for the first time: a foundational advance
✓ Expressed those 55,000 sequences in E.coli, with a sub-library for each target
✓ Created a genomic one-hybrid selection system sensitive enough to detect one hit in a million
✓ Found up to 15 novel zinc finger proteins

Biobricks and Protocols:

✓ Submitted 5 Biobricks to the registry, including our one-hybrid selection strain
✓ Created several chassis for our Biobricks, including our one-hybrid selection strain
✓ Used and shared our easy-to-follow protocols
✓ Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects

Human Practices

✓ Interviewed zinc finger researchers Dr. Keith Joung and Dr. George Church
✓ Researched intellectual property and how it applies to zinc finger proteins
✓ Created a timeline and case study of zinc finger intellectual property
✓ Handed out IP pamphlets and chassis data sheets at our poster presentation
✓ Educated local high school students about synthetic biology to provide accurate information from sources other than the media.


Our foundational advance is how we brought together novel technologies to create a pipeline that allows scientists to engineer novel interactions with DNA. This is a new way of making new parts: using selection on a computationally designed library. The genome is the next frontier in synthetic biology, and we want the iGEM and synthetic biology communities to use these techniques: we have provided detailed protocols on using lambda red and MAGE.
Retrieved from "http://2011.igem.org/Team:Harvard/Judging"