Team:Fatih Turkey/Notebook3

From 2011.igem.org

(Difference between revisions)
Line 617: Line 617:
     <div style="position: relative;">
     <div style="position: relative;">
                 <div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center ">
                 <div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center ">
-
            <p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p><p>asdada</p>
+
          <h4>30.07.11</h4><br>
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/a/a2/30%2C07%2C2011.pdf">https://static.igem.org/mediawiki/igem.org/a/a2/30%2C07%2C2011.pdf</a><br>
 +
<br>
 +
 
 +
<h4>31.07.11</h4><br>
 +
 
 +
<strong>PLASMID ISOLATION</strong><br>
 +
 
 +
-K(J04450)+Shuttle(K090403) 1/2/3<br>
 +
 
 +
-1002+1006+K<br><br>
 +
 
 +
&nbsp;
 +
 
 +
GLYCEROL STOCK<br>
 +
 
 +
-K+shuttle(K090403)<br>
 +
 
 +
-1006<br>
 +
 
 +
-1001<br>
 +
 
 +
-1001+1006+K<br>
 +
 
 +
-1002+1006+K<br>
 +
 
 +
-1004+1006+K<br>
 +
 
 +
-K143053+1006+K<br><br>
 +
 
 +
DIGASTION,LIGASTION (with pst and ecoR1)<br>
 +
 
 +
-1001+1005/shuttle<br>
 +
 
 +
-1002+1005/shuttle<br>
 +
 
 +
-1001+1006/shuttle<br>
 +
 
 +
-1002+1006/shuttle<br>
 +
 
 +
-K143053+1006/shuttle<br><br>
 +
 
 +
&nbsp;
 +
 
 +
TRANSFORMATION<br>
 +
 
 +
-1001+1005/shuttle<br>
 +
 
 +
-1002+1005/shuttle<br>
 +
 
 +
-1001+1006/shuttle<br>
 +
 
 +
-1002+1006/shuttle<br>
 +
 
 +
-K143053+1006/shuttle<br><br>
 +
 
 +
&nbsp;
 +
 
 +
LIQUID CULTURE<br>
 +
 
 +
-1002<br>
 +
 
 +
-1003<br>
 +
 
 +
-1004<br>
 +
 
 +
-1005<br>
 +
 
 +
we prepared those plasmids with amphiciline.<br><br>
 +
 
 +
&nbsp;
 +
 
 +
-1002+K<br>
 +
 
 +
-1003+K<br>
 +
 
 +
-1004+K<br>
 +
 
 +
-1005+K<br>
 +
 
 +
-1001+1005+K (only plasmid isolation)<br>
 +
 
 +
-1002+1005+k<br>
 +
 
 +
-1004+1005+K<br>
 +
 
 +
-1003+1006+K<br>
 +
 
 +
-J04500+1006+K<br>
 +
 
 +
we prepared those plasmids with chloramphenicol<br><br>
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/7/79/31%2C07%2C2011.pdf">https://static.igem.org/mediawiki/igem.org/7/79/31%2C07%2C2011.pdf</a><br>
 +
 
 +
<h1><strong>AUGUST 2011</strong></h1><br>
 +
 
 +
<h4><strong>01.08.11</strong></h4><br>
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/8/8e/01%2C08%2C2011.pdf">https://static.igem.org/mediawiki/igem.org/8/8e/01%2C08%2C2011.pdf</a><br>
 +
 
 +
 
 +
<h4><strong>07.08.11</strong></h4><br>
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/f/fa/07.08.11-_confirmation_digation.png">https://static.igem.org/mediawiki/igem.org/f/fa/07.08.11-_confirmation_digation.png</a><br>
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/2/2a/07.08.11.pdf">https://static.igem.org/mediawiki/igem.org/2/2a/07.08.11.pdf</a><br>
 +
<a href="https://static.igem.org/mediawiki/igem.org/2/2f/07%2C08%2C2011.pdf">https://static.igem.org/mediawiki/igem.org/2/2f/07%2C08%2C2011.pdf</a><br><br>
 +
 
 +
<h4><strong>08.08.11</strong></h4><br>
 +
 
 +
 
 +
<a href="https://static.igem.org/mediawiki/igem.org/4/46/08%2C08%2C2011.pdf">https://static.igem.org/mediawiki/igem.org/4/46/08%2C08%2C2011.pdf</a><br><br>
 +
 
 +
1001+K  C( C is the name in glycerol stock)<br>
 +
1002+K  C<br>
 +
1003+K  A<br>
 +
1004+K  A<br>
 +
1005+K  A<br>
 +
1001+1005/sht  BG<br>
 +
K143053+1006/sht  BG<br>                &gt;&gt;&gt;&gt;&gt;&gt;All of them have been isolated and digested with EcoR1, Pst1 then  made electrophoresis for confirmation.<br>
 +
 
 +
We have growned another some colonies from 1002+1006/sht and 1001+1006/sht (which grown in two antibiotics before) overnight (yesterday) and today they have been stroke which contain two antibiotics  in the morning and their falcons have been kept in the fridge .<br>
 +
 
 +
1002+1006/sht<br>     &gt;&gt;&gt;which picked colonies  x ,y ,z ,t   from A plate<br>
 +
&gt;&gt;&gt; which picked colonies   Ax , Bx, Cx , Dx , Ex  from B plate<br>
 +
 
 +
1001+1006/sht <br>    &gt;&gt;&gt;which picked colonies  Ax , Bx , Cx , Dx  from A plate<br>
 +
&gt;&gt;&gt; which picked colonies  x,y,z,t,s  from B plate<br><br>
 +
 
 +
&nbsp;
 +
 
 +
In the evening we observed  1001+1006/sht     By,  ADx , Bs streaks didn’t grow so we put in the new liquid culture from their morning’s liquid culture for glycerol stock. Also we prepared second  new liquid culture By for it’s plasmid isolation and confirmation.<br>
 +
 
 +
1002+1005/sht  C have been taken from glycerol stock and put in liquid culture for plasmid isolation. (it’s necessary for bacillus transformation to our DISC test)<br><br>
         </div>
         </div>
     </div>
     </div>

Revision as of 10:15, 28 October 2011

deneme baslik

Lab Garage

Notebook
Week:

30.07.11


https://static.igem.org/mediawiki/igem.org/a/a2/30%2C07%2C2011.pdf

31.07.11


PLASMID ISOLATION
-K(J04450)+Shuttle(K090403) 1/2/3
-1002+1006+K

  GLYCEROL STOCK
-K+shuttle(K090403)
-1006
-1001
-1001+1006+K
-1002+1006+K
-1004+1006+K
-K143053+1006+K

DIGASTION,LIGASTION (with pst and ecoR1)
-1001+1005/shuttle
-1002+1005/shuttle
-1001+1006/shuttle
-1002+1006/shuttle
-K143053+1006/shuttle

  TRANSFORMATION
-1001+1005/shuttle
-1002+1005/shuttle
-1001+1006/shuttle
-1002+1006/shuttle
-K143053+1006/shuttle

  LIQUID CULTURE
-1002
-1003
-1004
-1005
we prepared those plasmids with amphiciline.

  -1002+K
-1003+K
-1004+K
-1005+K
-1001+1005+K (only plasmid isolation)
-1002+1005+k
-1004+1005+K
-1003+1006+K
-J04500+1006+K
we prepared those plasmids with chloramphenicol

https://static.igem.org/mediawiki/igem.org/7/79/31%2C07%2C2011.pdf

AUGUST 2011


01.08.11


https://static.igem.org/mediawiki/igem.org/8/8e/01%2C08%2C2011.pdf

07.08.11


https://static.igem.org/mediawiki/igem.org/f/fa/07.08.11-_confirmation_digation.png
https://static.igem.org/mediawiki/igem.org/2/2a/07.08.11.pdf
https://static.igem.org/mediawiki/igem.org/2/2f/07%2C08%2C2011.pdf

08.08.11


https://static.igem.org/mediawiki/igem.org/4/46/08%2C08%2C2011.pdf

1001+K  C( C is the name in glycerol stock)
1002+K  C
1003+K  A
1004+K  A
1005+K  A
1001+1005/sht  BG
K143053+1006/sht  BG
                >>>>>>All of them have been isolated and digested with EcoR1, Pst1 then  made electrophoresis for confirmation.
We have growned another some colonies from 1002+1006/sht and 1001+1006/sht (which grown in two antibiotics before) overnight (yesterday) and today they have been stroke which contain two antibiotics  in the morning and their falcons have been kept in the fridge .
1002+1006/sht
     >>>which picked colonies  x ,y ,z ,t   from A plate
>>> which picked colonies   Ax , Bx, Cx , Dx , Ex  from B plate
1001+1006/sht 
    >>>which picked colonies  Ax , Bx , Cx , Dx  from A plate
>>> which picked colonies  x,y,z,t,s  from B plate

  In the evening we observed  1001+1006/sht     By,  ADx , Bs streaks didn’t grow so we put in the new liquid culture from their morning’s liquid culture for glycerol stock. Also we prepared second  new liquid culture By for it’s plasmid isolation and confirmation.
1002+1005/sht  C have been taken from glycerol stock and put in liquid culture for plasmid isolation. (it’s necessary for bacillus transformation to our DISC test)

2011 © Fatih Medical School

Retrieved from "http://2011.igem.org/Team:Fatih_Turkey/Notebook3"