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Team:Tokyo Metropolitan/Notebook/S13
From 2011.igem.org
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@transformation OG+O ② | @transformation OG+O ② | ||
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| + | = -Targetting- = | ||
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| + | @electrophoresis J23100-K317037, K283030, J22005<Br> | ||
| + | J23100-K317037, K283030 find binds.<Br> | ||
| + | J22005 not find bind.<Br> | ||
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| + | @gel extraction and confirmation binds by electrophoresis | ||
| + | J23100-K317037, K283030 | ||
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| + | @ligation J23100-K317037 and K283030 | ||
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| + | @transformation J23100-K317037-K283030 | ||
Revision as of 12:40, 5 October 2011
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-Visualization-
@confirmation of transformation (yesterday)
@restriction enzyme to pSB1C3(backbone for parts) (EcoRI, SpeⅠ)
@electrophore pSB1C3, O+G, total 4 samples.
@restriction enzyme to pSB1C3 (EcoRI, SpeⅠ)
@ligation OG+O ①
@transformation OG+O ①
@ligation OG+O ②
@transformation OG+O ②
-Targetting-
@electrophoresis J23100-K317037, K283030, J22005
J23100-K317037, K283030 find binds.
J22005 not find bind.
@gel extraction and confirmation binds by electrophoresis J23100-K317037, K283030
@ligation J23100-K317037 and K283030
@transformation J23100-K317037-K283030
