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Team:Tokyo Metropolitan/Notebook/S09
From 2011.igem.org
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Revision as of 10:23, 5 October 2011
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-Targeting-
-Plasmid extraction and DNA refining
Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.
Results measured concentration
No. 29 of J23100-K317037 was 150.
No. 19 of J23100-P0440 was 138.
J22005
I. 105
II. 155
III. 62
concentration was ng/ul
-Treat restriction enzyme
J23100-K317037 -> S and P
J23100-P0440 -> E and P
K283030 -> X and P
J22005 -> N
pSB1C3 -> E and P
incubated at 37 deg C after treat with the preceding enzymes.
-Setrilization for liquid medium
Setrilised for nine tubes 4 ml.
