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Team:Tokyo Metropolitan/Notebook/S05
From 2011.igem.org
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Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ. | ||
Revision as of 09:18, 5 October 2011
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-Speeding up-
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
