Team:KAIT Japan/Project

From 2011.igem.org

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== '''Colony on Colony''' ==
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== '''Intro''' ==
     We tried to overlap colony on colony. Generally, colony and colony don’t overlap and approach each other, because of the cell-cell communications. Bacterias also have a system, so called quorum sensing, to control of production of signal chemicals depend on the bacteria’s density.
     We tried to overlap colony on colony. Generally, colony and colony don’t overlap and approach each other, because of the cell-cell communications. Bacterias also have a system, so called quorum sensing, to control of production of signal chemicals depend on the bacteria’s density.
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=== Part 2 ===
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=== Quorum Sensing ===
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Quorum sensing is a system of stimulus and response correlated
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to population density. Many species of bacteria use quorum
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sensing to coordinate gene expression according to the density of
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their local population. In similar fashion, some social insects use
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quorum sensing to determine where to nest.
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=== AHL ===
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=== The Experiments ===
 
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=== AiiA ===
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=== Part 3 ===
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== Results ==
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== XXX ==
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Revision as of 15:49, 4 October 2011

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Contents

Intro

    We tried to overlap colony on colony. Generally, colony and colony don’t overlap and approach each other, because of the cell-cell communications. Bacterias also have a system, so called quorum sensing, to control of production of signal chemicals depend on the bacteria’s density.

    We pay attention quorum sensing system. Bacterias produce and secret autoinducer against other bacterias by quorum sensing system, as a result of the system, bacteria senses other bacterias. Most of Gram-negative bacterias make use of autoinducer named AHL. We applied E. coli to our experiment and we use aiia which is catabolic enzyme to inhibit AHL. We tried to make a recombinant bacteria to cancelled sensing of other bacterias and expected to form the colony of bacterias on the other bacterias.




Project Details

Quorum Sensing

Quorum sensing is a system of stimulus and response correlated to population density. Many species of bacteria use quorum sensing to coordinate gene expression according to the density of their local population. In similar fashion, some social insects use quorum sensing to determine where to nest.


AHL

AiiA

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