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Team:Osaka/week7
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
| - | ==September 11(Sun)== | + | ==September 11 (Sun)== |
# Gel extraction of digests | # Gel extraction of digests | ||
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
| Line 15: | Line 15: | ||
| - | ==September 12(Mon)== | + | ==September 12 (Mon)== |
# To liquid culture:103,104,105,106 | # To liquid culture:103,104,105,106 | ||
| - | ==September 13(Tue)== | + | ==September 13 (Tue)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 27: | Line 27: | ||
# Transformation of ligation products | # Transformation of ligation products | ||
| - | ==September 14(Wed)== | + | ==September 14 (Wed)== |
# To liquid culture:107,108 | # To liquid culture:107,108 | ||
| - | ==September 15(Thu)== | + | ==September 15 (Thu)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 36: | Line 36: | ||
# To liquid culture:108 | # To liquid culture:108 | ||
| - | ==September 16(Fri)== | + | ==September 16 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 42: | Line 42: | ||
| - | ==September 17(Sat)== | + | ==September 17 (Sat)== |
# Ligation | # Ligation | ||
#* 109(K):1-2M(upstream)+101(downstream)+1-5A(vector) | #* 109(K):1-2M(upstream)+101(downstream)+1-5A(vector) | ||
Revision as of 13:51, 2 October 2011
Contents |
September 11 (Sun)
- Gel extraction of digests
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
- Transformation of ligation products
September 12 (Mon)
- To liquid culture:103,104,105,106
September 13 (Tue)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 107(A):12(upstream)+103(downstream)+1-1G(vector)
- 108(A):12(upstream)+104(downstream)+1-1G(vector)
- Transformation of ligation products
September 14 (Wed)
- To liquid culture:107,108
September 15 (Thu)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:108
September 16 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
September 17 (Sat)
- Ligation
- 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
- 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
- 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
- 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
- Transformation of ligation products
