Team:Minnesota/davis950-08032011164451-14

From 2011.igem.org

(Difference between revisions)
m (Automatic page editing)
m (Automatic page editing)
 
Line 1: Line 1:
__NOTOC__
__NOTOC__
{{Team:Minnesota/Top}}
{{Team:Minnesota/Top}}
-
<br>== Digest of ho1 and Cph8 ==
+
<br>
 +
== Digest of ho1 and Cph8 ==
'''Project:''' Regulatory
'''Project:''' Regulatory
Line 32: Line 33:
<nowiki>Digestion should be run for 24 hours and quenched by heating at 65 degree C for 20 minutes.</nowiki>
<nowiki>Digestion should be run for 24 hours and quenched by heating at 65 degree C for 20 minutes.</nowiki>
 +
{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Latest revision as of 22:46, 27 September 2011

Mnlogo.jpg
Home Team Project Software Protocols Notebook Attributions Safety

Digest of ho1 and Cph8

Project: Regulatory

Date: Wed, 03 Aug 2011 16:44:51 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Wed, 03 Aug 2011 16:47:49 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Double digest of ho1 and Cph8 set up and placed in 37 degree C incubator. Reaction mixture was as follows:


  • 3 ul NEBuffer 3 (100% activity for both BglII and NotI)
  • 1 ul BglII
  • 1 ul NotI (acquired from Claudia's lab)
  • 10 ul template DNA
  • 16 ul ddH2O


Digestion should be run for 24 hours and quenched by heating at 65 degree C for 20 minutes.


240px Cbs wdmk blk d2d.jpg
BTIheader white.jpg
Retrieved from "http://2011.igem.org/Team:Minnesota/davis950-08032011164451-14"