Team:Colombia/Notebook/Protocols

From 2011.igem.org

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{| border="1" align="left" style="text-align:center;"
 +
|Reactives
 +
|1X µl
 +
|3X µl
 +
|H2O
 +
|18.8
 +
|56.4
 +
|Buffer PFX
 +
|2.5
 +
|7.5
 +
|MgSO4
 +
|0.5
 +
|1.5
 +
|dNTP´s
 +
|0.75
 +
|2.25
 +
|FW (Pr3)
 +
|0.75
 +
|2.25
 +
|RV (Pr4)
 +
|0.75
 +
|2.25
 +
|PFX
 +
|0.2
 +
|0.6
 +
|ADN
 +
|0.75
 +
|2.25
 +
|Mix Volume
 +
|25
 +
|75
 +
|}

Revision as of 16:34, 27 September 2011

Template:Https://2011.igem.org/User:Tabima

Protocols

PCR for 16s (10 µl)

Reactives µl µl
Buffer 2.5 1
MgCl2 2 0.8
DNTP´s 0.5 0.2
P1492R 0.5 0.2
P27F 0.5 0.2
Taq 0.25 0.1
DNA 2 0.8
H2O 17.75 6.8
Final Vol 26 10















PCR Pfx

Reactives ul
H2O 17.2
Buffer PFX 3
MgSO4 0.6
dNTP´s 0.9
FW 0.9
RV 0.9
Enhancer 3
Taq PFX 0.5
ADN 3
Total Mix 30















Reactives 1X µl 3X µl H2O 18.8 56.4 Buffer PFX 2.5 7.5 MgSO4 0.5 1.5 dNTP´s 0.75 2.25 FW (Pr3) 0.75 2.25 RV (Pr4) 0.75 2.25 PFX 0.2 0.6 ADN 0.75 2.25 Mix Volume 25 75
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