Data For Our Favorite New Parts

BBa_K516210

BBa_K516210 (p_Tet-RBS30-LuxI) - HSL synthesis device, aTc inducible.
This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.

BBa_K516334

BBa_K516334 (p_Lambda-RBS30-LuxR-T-p_Lux-RBS34-mRFP-TT) - 3OC₆-HSL biosensor, mRFP output
This is one of the four biosensors (different RBSs) built and characterized. This measurement system senses the HSL concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.

BBa_K516230

BBa_K516230 (p_Tet-RBS34-mRFP-TT) - aTc inducible, mRFP output
This is one of the four p_Tet measurement systems (different RBSs) built and characterized. This measurement system senses the aTc concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.

Data For existing Parts

RBSs from the community collection

• Experience page of BBa_B0030
• Experience page of BBa_B0031
• Experience page of BBa_B0032
• Experience page of BBa_B0034

RBSs were used for the fine tuning of CTRL+E. Different experimental conditions were assayed.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:



Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA or LuxI:

BBa_K081022

• Experience page of BBa_K081022

This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).

The Hill's estimated parameters are reported in the table below:

RBS	αpLux [(AUr/min)/cell]	δpLux [-]	ηpLux [-]	kpLux [ng/ml]
BBa_B0030	438 [10]	0.05 [>100]	2 [47]	1.88 [27]
BBa_B0031	9.8 [7]	0.11 [57]	1.2 [29]	1.5 [26]
BBa_B0032	206 [3]	0 [>>100]	1.36 [10]	1.87 [9]
BBa_B0034	1105 [6]	0.02 [>100]	1.33 [19]	2.34 [18]

The operative parameters are reported in the table below:

RBS	RPUmax	RPUmin	Switch point [nM]	Linear boundaries [MIN; MAX] [nM]
B0030	4.28	0.20	1.08	[0.36; 3.27]
B0031	4.93	0.55	0.25	[0.03; 2.30]
B0032	9.49	0.02	0.47	[0.07; 3.07]
B0034	21.53	0.51	0.53	[0.08; 3.77]

BBa_R0040

• Experience page of BBa_R0040

pTet promoter was characterized with various RBSs and mRFP.

The Hill's estimated parameters are reported in the table below:

RBS	αpTet [(AUr/min)/cell]	δpTet [-]	ηpTet [-]	kpTet [nM]
BBa_B0030	230.67 [3.7]	0.028 [91.61]	4.61 [23.73]	8.75 [4.16]
BBa_B0031	ND	ND	ND	ND
BBa_B0032	55.77 [12]	1.53E-11 [>>100]	4.98 [57.62]	7.26 [14.98]
BBa_B0034	120 [5.95]	0.085 [40.6]	24.85 [47.6]	9 [5.43]

Data are provided as average [CV%]

The operative parameters are reported in the table below:

RBS	RPUmax	RPUmin	Switch point [ng/ml]	Linear boundaries [MIN; MAX] [ng/ml]
B0030	1.53	~0	7.95	[4.66;11.99]
B0031	ND	ND	ND	ND
B0032	3.16	~0	6.7	[4.45;10.05]
B0034	2.73	0.23	8.96	[8.27;9.71]

Improved existing parts

BBa_K516999

• BBa_K516999

This vector was designed and realized in order to facilitate the cloning of coding sequences downstream of the strong promoter p_Tet.

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