Team:UNIPV-Pavia/Parts/DataPage

From 2011.igem.org

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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a> (p<sub>Tet</sub>-RBS30-LuxI) - HSL synthesis device, aTc inducible. <br> This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.
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<A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K516210">BBa_K516210</a> (p<sub>Tet</sub>-RBS30-LuxI) - HSL synthesis device, aTc inducible. <br> This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.
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<li><a href='http://partsregistry.org/Part:BBa_B0030:Experience'>BBa_B0030</a>  
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<li><a href='http://partsregistry.org/Part:BBa_B0030:Experience'>Experience page of BBa_B0030</a>  
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<li><a href='http://partsregistry.org/Part:BBa_B0031:Experience'>BBa_B0031</a>
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<li><a href='http://partsregistry.org/Part:BBa_B0031:Experience'>Experience page of BBa_B0031</a>
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<li><a href='http://partsregistry.org/Part:BBa_B0032:Experience'>BBa_B0032</a>
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<li><a href='http://partsregistry.org/Part:BBa_B0032:Experience'>Experience page of BBa_B0032</a>
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<li><a href='http://partsregistry.org/Part:BBa_B0034:Experience'>BBa_B0034</a>
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<li><a href='http://partsregistry.org/Part:BBa_B0034:Experience'>Experience page of BBa_B0034</a>
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K081022</b> </span></h2></div></nowiki>
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<li><a href='http://partsregistry.org/Part:BBa_K081022:Experience'>Experience page of BBa_K081022</a>
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This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).
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<div align="right"><small><a href="#indice">^top</a></small></div><br><br>
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<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K081022</b> </span></h2></div></nowiki>  
<nowiki><div class="art-postcontent"><h2><span class="mw-headline"> <b>BBa_K081022</b> </span></h2></div></nowiki>  

Revision as of 16:06, 21 September 2011

UNIPV TEAM 2011

Data Page

Data For Our Favorite New Parts



BBa_K516210





BBa_K516210 (pTet-RBS30-LuxI) - HSL synthesis device, aTc inducible.
This is one of four systems with different RBSs (output modulation) tested to quantify the produced HSL. The results obtained by testing this part allowed the identification of important model parameters to predict the behavior of the whole CTRL+E circuit.



BBa_K516334



BBa_K516334 (pLambda-RBS30-LuxR-T-pLux-RBS34-mRFP-TT) - 3OC6-HSL biosensor, mRFP output
This is one of the four biosensors (different RBSs) built and characterized. This measurement system senses the HSL concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.



BBa_K516230





BBa_K516230 (pTet-RBS34-mRFP-TT) - aTc inducible, mRFP output
This is one of the four pTet measurement systems (different RBSs) built and characterized. This measurement system senses the aTc concentration in the medium and responds with an RFP output. Several RBSs have been used to modulate the output range. This is a simple measurement system, used to identify important model parameters used to simulate the behavior of the whole circuit.



Data For existing Parts



RBSs from the community collection




RBSs were used for the fine tuning of CTRL+E. Different experimental conditions were assayed.

Estimated efficiencies in pSB4C5 plasmid with -RBSx-mRFP-TT coding sequence under the control of the specified promoter:

RBS effpLux effpTet effJ23101 Declared efficiency
B00300.401.68142.450,6
B00310.01ND0.040,07
B00320.190.41930.400,3
B00341111


Estimated efficiencies in pSB4C5 plasmid with pTet-RBSx-GeneX-TT, with GeneX=mRFP, AiiA or LuxI:

RBS effmRFP effAiiA effLuxI Declared efficiency
B00301.720.530.450,6
B00310.030.830.0280,07
B00320.370.50N.D.0.3
B00341111

BBa_K081022



This device has been characterized with several RBSs in order to modulate the output range. In this way, this device can be used to tightly control the expression of downstream encoded protein from a very weak production (using weak RBS) to a very intensive production (with strong RBSs).



BBa_K081022





We've Also Characterized the Following Parts





Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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