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| This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. | | This instrument allows long incubation of the microplate, the chance to set the shaking movements, the duration of all operations, including cycles, and the dispensation of liquid material in the wells. These actions are managed by a user interface operated by i-Control software, ranging from scheduling experiments to the cleaning of injectors. |
| We used this instrument for the following measurement techniques: </p> | | We used this instrument for the following measurement techniques: </p> |
- | <p></p> | + | <div class="listcircle"> |
- | <ul>
| + | <div style="text-align:justify"> |
- | <li> fluorescence intensity; </li>
| + | <div class="art-postcontent" style="margin-left: 15pt;"> |
| + | <li> fluorescence intensity; </li> |
| <li> absorbance. </li> | | <li> absorbance. </li> |
- | </ul></td> | + | </div></div></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |
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| <a name="Nanodrop_ND_1000_spectrophotometer"></a> | | <a name="Nanodrop_ND_1000_spectrophotometer"></a> |
- | <h2> <span class="mw-headline">Nanodrop ND 1000 spectrophotometer</span></h2> | + | <h2> <span class="mw-headline">NanoDrop ND 1000 spectrophotometer</span></h2> |
| <table width="100%"> | | <table width="100%"> |
| <tbody> | | <tbody> |
| <tr> | | <tr> |
| <td align="left" valign="top"><p></p> | | <td align="left" valign="top"><p></p> |
- | <p align="justify"> Nanodrop is a really helpfull instrument used to measure concentration of DNA in a micro-volume of sample and OD in cell cultures.</p> | + | <p align="justify"> The Thermo Scientific NanoDrop™ 1000 Spectrophotometer (v 3.7) is a full-spectrum (220 - 750nm) spectrophotometer. This instrument is a really helpfull instrument that elimante the needs of cuvettes and is capable to measure highly concentrated samples without dilution. We use NanoDrop to measure: |
- | <p></p></td> | + | <div class="listcircle"> |
| + | <div style="text-align:justify"> |
| + | <div class="art-postcontent" style="margin-left: 15pt;"> |
| + | <li> concentration of DNA in a micro-volume of sample (1 microliter);</li> |
| + | <li> and OD in cell cultures (2 microliter).</li></p> |
| + | <p></p></div></div></td> |
| <td align="right" valign="top" width="20%"><table border="0"> | | <td align="right" valign="top" width="20%"><table border="0"> |
| <tbody> | | <tbody> |